Production of Soluble Proteins in Periplasm of Escherichia Coli - Dr R. Nagasundara Ramanan - Online
Date From: 19 April 2017
Description: An online presentation by Dr R. Nagasundara Ramanan (Monash University Malaysia) organised by Biochemical Engineering Special Interest Group.
Event Type: Webinar
Venue: Online 17:00-18:00 MYT
Organiser: Biochemical Engineering Special Interest Group
Escherichia coli is the most widely used expression system for non-glycosylated recombinant proteins for both analytical and preparative purposes. To facilitate extraction of the overexpressed recombinant protein in soluble form and to maintain the biological activity, the protein is usually targeted to the periplasm. In order to increase yield of the recombinant protein, several strategies such as codon optimization, signal sequence tagging and bacterial strain engineering have been developed. However, all these strategies involve series of trial and error steps, and often may not be successful. To overcome this tedious trial and error process, we have utilized bioinformatics tools to develop a web based tool. The tool PERISCOPE is freely available for the researcher to predict the level of protein expressed based on the combination of signal sequence and amino acid sequence of a protein. This online tool is first of its kind to predict the protein in periplasm of E. coli. The talk will cover the systematic methodology that we adopt to develop this tool and also other challenge that impact the recovery of proteins from periplasm of E. coli.
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Dr R. Nagasundara Ramanan is a Senior Lecturer in Chemical Engineering Discipline, School of Engineering, Monash University Malaysia. Prior to join Monash in November 2010, he worked as a postdoctoral fellow in Universiti Putra Malaysia (UPM) where he has to lead a group of postgraduate students for their research work. Dr. Ramanan did his Chemical Engineering in India and did his PhD in Bioprocess Engineering from UPM. He has a decade of multi-national industrial experience in petro-chemical sector before continuing his study in UPM. Dr. Ramanan is an active researcher in the area of Bioprocess and has been collaborating with different discipline researchers from national and international experts. He has received grants from Ministry of Higher Education (MOHE) and Ministry of Science, Technology and Innovation (MOSTI), Malaysia. Besides he and his team received fellowship from Australian government to carry out some part of their research in Australia and Gates Foundation grant to visit MIT. He has published more than 50 international journal articles. He is currently heading the Malaysian chapter of IChemE Biochemical Engineering Special Interest Group.
A one-hour online session: 40 minutes' presentation + 20 minutes' Q&A.
Duration (for CPD recording purposes): up to one hour.
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This webinar is free of charge and open to all to attend, but if you wish to access the slides and a recording to replay on demand then you'll need to be a member of the Biochemical Engineering Special Interest Group.
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