Overview

Protein crystallization is an emerging purification technology in the downstream processing of therapeutic proteins, where the state-of-the-art purification technology is chromatography. Using bovine serum albumin (BSA) as the impurity protein and lysozyme as the model protein, experimental results showed that the impurity protein could raise the solubility of the model protein and hence reduce the recovery yield of crystallization in agreement with previous study. The non-specific protein-protein interactions between lysozyme and BSA under different conditions were studied with small angle X-ray scattering (SAXS) to identify conditions that minimized the effects of impurity (i.e. BSA) on the solubility of lysozyme.

Speaker

Dr Chen Wenqian, Assistant Professor, National University of Singapore

Chen obtained his MEng and PhD in chemical engineering from Imperial College London. Chen specializes in protein and peptide crystallization for purification and formulation purposes.